名称：重组人TARC/CCL17

Species：9

Accession：Q92583

GeneID：6361

Source：Escherichia coli.

Molecular Weight：Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids.

Quantity：5μg/20μg/1000μg

AA Sequence：ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S

Purity：> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity：Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.

Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation：Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Endotoxin：Less than 1 EU/μg of rHuTARC/CCL17 as determined by LAL method.

Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reference：1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308.
2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21.
3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21.
4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8.
5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8.

Background：Human CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16 in humans. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309.