名称:重组人MIP-3/CCL23
Synonyms:CKB-8, MIP-3, MIPIF-1
Species:9
Accession:P55773
GeneID:6368
Source:Escherichia coli.
Molecular Weight:Approximately 11.4 kDa, a single, non-glycosylated polypeptide chain containing 99 amino acids.
Quantity:5μg/20μg/1000μg
AA Sequence:RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
Purity:> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin:Less than 1 EU/μg of rHuMIP-3/CCL23 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference:1. Novak H, Muller A, Harrer N, et al. 2007. J Immunol, 178: 4335-41.
2. Votta BJ, White JR, Dodds RA, et al. 2000. J Cell Physiol, 183: 196-207.
3. Poposki JA, Uzzaman A, Nagarkar DR, et al. 2011. J Allergy Clin Immunol, 128: 73-81 e4.
Background:CCL23 is a member of CC chemokine family and is encoded by CCL23 gene located on Chr.17 in humans, where near several other CC chemokines. Highly expressed in ***** lung, liver, skeletal muscle and pancreas, this protein shows strong chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. It elicits the effects by binding to CCR1. CCL23 is reported that it can be cleaved into four forms: CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99).
SDS-PAGE: