名称：重组人Eotaxin-2/CCL24

Synonyms：CK-beta-6, MPIF-2, Small-inducible Cytokine A24

Species：9

Accession：O00175

GeneID：6369

Source：Escherichia coli.

Molecular Weight：Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.

Quantity：5μg/20μg/1000μg

AA Sequence：VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA

Purity：> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity：Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-100 ng/ml.

Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation：Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin：Less than 1 EU/μg of rHuEotaxin-2/CCL24 as determined by LAL method.

Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reference：1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5.
2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79.
3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14.

Background：CCL24 is a secreted protein, encoded by CCL24 gene in humans located on Chr.7, and produced by activated monocytes and T lymphocytes. CCL24 has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.