名称:rHBsAg-preS2
Source:Escherichia coli.
Molecular Weight:Approximately 5.7 kDa, a single non-glycosylated polypeptide chain containing 55 amino acids.
Quantity:10μg/50μg/1000μg
AA Sequence:MQWNSTTFHQ ALLDPKVRGL YFPAGGSSSG TVNPVPTTAS PISSIFSRTG DPAPN
Purity:>95% by SDS-PAGE and HPLC analyses.
Applications:1. Immunochromatography (capture and conjugate);
2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS2;
3. ELISA.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 50mM NaCl.
Endotoxin:Less than 1 EU/μg of rHBsAg-preS2 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.