名称:重组大鼠LIX/CXCL5
Synonyms:Cytokine LIX, Small-inducible Cytokine B5
Accession:P97885
GeneID:60665
Source:Escherichia coli.
Molecular Weight:Approximately 10.1 kDa, a single, non-glycosylated polypeptide chain containing 93 amino acids.
Quantity:5μg/20μg/1000μg
AA Sequence:APFSAMVATE LRCVCLTLAP RINPKMIANL EVIPAGPHCP KVEVIAKLKN QKDNVCLDPQ APLIKKVIQK ILGSENKKTK RNALALVRSA STQ
Purity:> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin:Less than 1 EU/μg of rRtLIX/CXCL5 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference:1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7.
Background:CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. The expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-γ. Recombinant rat CXCL5 contains 93 amino acids which is a single, non-glycosylated polypeptide chain. Rat CXCL5 shares 57 % amino acid sequence identity with human CXCL5.
SDS-PAGE: