名称:重组大鼠IP-10/CXCL10
Synonyms:Gamma-IP10, Small-inducible Cytokine B10, Protein Mob-1
Accession:P48973
GeneID:245920
Source:Escherichia coli.
Molecular Weight:Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.
Quantity:5μg/25μg/1000μg
AA Sequence:IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAP
Purity:> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50 ng/ml.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin:Less than 1 EU/μg of rRtIP-10/CXCL10 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.