名称：重组大鼠SDF-1α/CXCL12α

Accession：Q9QZD1

GeneID：24772

Source：Escherichia coli.

Molecular Weight：Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Quantity：2μg/10μg/1000μg

AA Sequence：KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

Purity：> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity：Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.

Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation：Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin：Less than 1 EU/μg of rRtSDF-1α/CXCL12α as determined by LAL method.

Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reference：1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.
2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.
3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.
4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.
5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.
6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.
7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.

Background：CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α.